Open AccessEffect of Basal Media and Sugar Types on in Vitro Regeneration of Grammatophyllum speciosum Blume
Author(s) -
Montakarn Pimsen,
Kamnoon Kanchanapoom
Publication year - 2011
Publication title -
notulae scientia biologicae
Language(s) - English
Resource type - Journals
eISSN - 2067-3264
pISSN - 2067-3205
DOI - 10.15835/nsb336153
Subject(s) - sucrose , sorbitol , mannitol , explant culture , sugar , glycerol , murashige and skoog medium , chemistry , botany , food science , browning , horticulture , in vitro , biology , biochemistry
Protocorms of Grammatophyllum speciosum Blume. were initiated from immature seeds on solid MS medium containing 15% (v/v) coconut water (CW) and 3% (w/v) sucrose. Protocorms, 2-4 mm in length were used as explants and subcultured to MS and VW (Vacin and Went, 1949) media containing 15% (v/v) CW and 2 or 3% (w/v) sucrose. Protocorms gave the highest formation of PLBs (protocorm-like bodies) at 3.1 PLBs/explant on MS solid medium containing 15% CW and 2% sucrose. For the test with different carbon sources, protocorms were cultured in liquid MS medium supplemented with 4 kinds of sugar, namely sucrose, glucose, sorbitol and mannitol at 2, 4, 6 or 8% (w/v) and cultured for 1, 2, 3 or 4 weeks. They were then transferred to MS solid medium containing 15% CW and 2% sucrose. Results revealed that after 4 weeks in MS liquid medium, sucrose and glucose had an inhibitory effect and 8% glucose gave a high percentage of protocorm browning. In contrast, sorbitol and mannitol were effective for protocorm regeneration and both sugar alcohols had a positive effect on the formation of PLBs and the development of PLBs into plantlets.