Open AccessCryopreservation of aromatic ginger Kaempferia galanga L. by encapsulation-dehydration
Author(s) -
T. S. Preetha,
A. S. Hemanthakumar,
P. N. Krishnan
Publication year - 2021
Publication title -
notulae scientia biologicae
Language(s) - English
Resource type - Journals
eISSN - 2067-3264
pISSN - 2067-3205
DOI - 10.15835/nsb13411024
Subject(s) - shoot , rhizome , zingiberaceae , dehydration , chemistry , sucrose , horticulture , callus , botany , murashige and skoog medium , biology , in vitro , tissue culture , food science , biochemistry
Kaempferia galanga L. is an endangered multi-purpose medicinal plant in Family Zingiberaceae, the rhizomes of which are used for several ayurvedic formulations. Encapsulation-dehydration (ED) method was optimized for cryopreservation of shoot tips of K. galanga. Shoot tips (STs) bearing the apical meristem dissected from the established in vitro shoot cultures were preconditioned in MS+0.4 M sucrose prior to encapsulation in calcium alginate and the beads subsequently transferred to MS liquid+0.3 M sucrose for 3 days afterward dehydration inside the laminar airflow for 4 hours upon rapid freezing in LN and rapid thawing produced maximum 62.2% survival and 46.7% regeneration rates. Shoot regeneration was observed from the apical meristems exclusive of intermediary callus phase. The plantlets regenerated from cryopreserved STs transferred to the field were phenotypically analogous with the mother plant.