Open AccessComparative analysis of genetic diversity in Norway spruce (Picea abies) clonal seed orchards and seed stands
Author(s) -
Elena Ciocîrlan,
N. Şofletea,
Georgeta Mihai,
Marius Teodosiu,
Alexandru Lucian Curtu
Publication year - 2021
Publication title -
notulae botanicae horti agrobotanici cluj-napoca
Language(s) - English
Resource type - Journals
eISSN - 1842-4309
pISSN - 0255-965X
DOI - 10.15835/nbha49412575
Subject(s) - picea abies , genetic diversity , biology , seed orchard , botany , microsatellite , karst , allele , population , paleontology , biochemistry , demography , sociology , gene
Norway spruce, Picea abies (L.) Karst. is the most important conifer species in Romania and the most planted tree species in the Carpathian Mountains. Here we compare the genetic diversity of four Norway spruce clonal seed orchards and two seed stands located in the Eastern Carpathians. A set of highly polymorphic nuclear microsatellite markers was used. The analysis of genotypic identity of ramets for each Norway spruce clone in all seed orchards indicated that nearly all sampled ramets (97%) were genetically identical. The genetic diversity in seed orchards (He=0.700) was slightly smaller compared to the seed stands (He=0.718). Allelic richness was higher in seed stands (10.874), compared to clonal seed orchards (8.941). The Bayesian analysis indicated a genetic structure with two clusters, one corresponding to the clonal seed orchards and a second one consisting of the two seed stands. Our results provide valuable information for the management of Norway spruce seed orchards in Romania.