Open AccessComparative Polyphenolic Content and Antioxidant Activities of Some Wild and Cultivated Blueberries from Romania
Author(s) -
Andrea Bunea,
Olivia Dumitriţa Rugină,
Adela Pintea,
Zoriţa Sconţa,
Claudiu Ioan Bunea,
Carmen Socaciu
Publication year - 2011
Publication title -
notulae botanicae horti agrobotanici cluj-napoca
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.332
H-Index - 32
eISSN - 1842-4309
pISSN - 0255-965X
DOI - 10.15835/nbha3926265
Subject(s) - oxygen radical absorbance capacity , chemistry , petunidin , polyphenol , dpph , anthocyanin , food science , abts , vaccinium myrtillus , trolox equivalent antioxidant capacity , vaccinium , berry , botany , antioxidant , trolox , ferric reducing ability of plasma , cyanidin , malvidin , antioxidant capacity , biochemistry , biology
Two wild and three cultivated blueberry varieties (‘Elliot’, ‘Bluecrop’ and ‘Duke’) from Romania were analyzed comparatively in order to determine the total polyphenols, total anthocyanins, total flavonoids content and measuring the antioxidant activity using three different single electron transfer-based assays, Trolox equivalent antioxidant capacity (ABTS), ferric reducing ability (FRAP), 2,2-diphenylpicrylhydrazil radical scavenging capacity (DPPH) and one hydrogen atom transfer-assay, oxygen radical absorbance capacity (ORAC). Total polyphenols content ranged from 424.84 - 819.12 mg GAE/100 g FW, total flavonoids ranged from 84.33-112.5 mg QE /100 g FW and total anthocyanins ranged from 100.58-300.02 C3GE/100g FW. The anthocyanins were separated and quantified using RP-HPLC-DAD. In Vaccinium myrtillus, petunidin-3-glucoside and delphinidin-3-glucoside have the highest contribution to the anthocyanin content while in Vaccinium corymbosum, peonidin-3-galactoside represent the major anthocyanin. Except for ORAC assay (r=0.765), all antioxidant activity values obtained were highly correlated with total polyphenol content (0.923≤ r ≤0.986). Wild blueberries had higher total polyphenols content and also antioxidant activity compared with cultivated ones.