Open AccessThe Rate of Success of the Accelerated Solvent Extraction (Ase) of Fat and Organochlorine Pesticides from Dried Fish Meat Samples
Author(s) -
Ana Andreea Cioca,
Olaf Heemken,
Marian Mihaiu
Publication year - 2017
Publication title -
bulletin of university of agricultural sciences and veterinary medicine cluj-napoca. veterinary medicine
Language(s) - English
Resource type - Journals
eISSN - 1843-5378
pISSN - 1843-5270
DOI - 10.15835/buasvmcn-vm:12589
Subject(s) - chemistry , chromatography , extraction (chemistry) , accelerated solvent extraction , analyte , pesticide , polychlorinated biphenyl , solvent , ethyl acetate , fish <actinopterygii> , sample preparation , gas chromatography , environmental chemistry , fishery , biology , organic chemistry , agronomy
The replacement of conventional sample preparation techniques with newer techniques which are automated, faster and more eco-friendly, is nowadays desired in every analytical laboratory. One of the techniques with the attributes mentioned above is the Accelerated Solvent Extraction. In order to evaluate how successful this method is for the extraction of fat and organochlorine pesticides (OCPs) from dried fish meat samples, we have tested two series of diverse fish using Dionex™ 350 ASE provided by Thermo Scientific™ (Germany). For a more interesting approach, we added to our investigation 7 polychlorinated biphenyl (PCBs), 3 thricholorobenzenes, 2 tetrachlorobenzenes, 1 pentachlorobenzenes and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The study focused on comparing the recoveries of these analytes from different fish samples, after replacing the conventional reference method of the laboratory with ASE. The ASE parameters tested were previously used for the extraction of fat and polybrominated diphenyl ethers (PBDE) from fish samples: temperature: 120 ° C; static time: 5 min; number of cycles: 3; flushing volume: 25%; rinse with nitrogen: 90 s; solvent: cyclohexane/ethyl acetate (ratio 1:1). The ASE method provided similar and in some cases better results when compared to the standard reference method, more rapidly, eco-friendly and safer. Any high or low recoveries of the analytes taken into study are attributed to random or systematic errors during the Clean-up step of the extracts and the quantification with Gas Chromatography coupled with Tandem Mass-Spectrometry (GC MS/MS).