Open AccessUsing Bioinformatics Tools for Identification and Characterization of Transcriptome Derived EST-SSRs in Silver Fir (Abies alba Mill.)
Author(s) -
Dragoș Postolache
Publication year - 2017
Publication title -
bulletin of university of agricultural sciences and veterinary medicine cluj-napoca. horticulture
Language(s) - English
Resource type - Journals
eISSN - 1843-5394
pISSN - 1843-5254
DOI - 10.15835/buasvmcn-hort:12625
Subject(s) - transcriptome , microsatellite , biology , abies alba , identification (biology) , computational biology , genetics , gene , botany , gene expression , allele , picea abies
Bioinformatics tools have been used to evaluate silver fir de novo assembled 454 transcriptome. A total of 3500 EST-SSRs were detected in the 454 transcriptome of silver fir. Most abundant are tri-nucleotide SSRs being followed by tetra- SSRs and di- SSRs. In addition, we determined the density, frequency, average length and average repeat number of EST-SSRs in the 454 transcriptome of silver fir.