Open AccessThe Effect of pH Stress on the Survival of Rhizobium Trifolii and Sinorhizobium Meliloti in Vitro
Author(s) -
Monica Niste,
Roxana Vidican,
Ioan Rotar,
Rodica Pop
Publication year - 2013
Publication title -
bulletin of university of agricultural sciences and veterinary medicine cluj-napoca. agriculture
Language(s) - English
Resource type - Journals
eISSN - 1843-5386
pISSN - 1843-5246
DOI - 10.15835/buasvmcn-agr:9811
Subject(s) - rhizobia , sinorhizobium meliloti , sinorhizobium , rhizobium , mannitol , rhizobiaceae , biology , agar , bacteria , nitrogen fixation , food science , symbiosis , chemistry , biochemistry , genetics
Nitrogen-fixing symbiotic bacteria known as rhizobia can exist in different soils and adapt to different environmental conditions. The aim of this study was to determine the impact of pH on the growth of Rhizobium trifolii and Sinorhizobium meliloti. Rhizobial species were isolated using yeast extract mannitol agar medium) in which the pH values were adjusted to 5.0, 6.0, 8.0 and 9.0 by adding HCl and NaOH. The optimum pH for rhizobia is neutral or slightly alkaline (pH 8) and they are more sensitive to acidity. Sinorhizobium meliloti developed better in an acid medium compared to Rhizobium trifolii.