Open AccessAnalytical Determination of Carotenoids in Inland Ocimum Basilicum L.
Author(s) -
Cristian Matea,
Maria-Loredana Soran,
Adela Pintea,
Constantin Bele
Publication year - 2010
Publication title -
bulletin of university of agricultural sciences and veterinary medicine cluj-napoca. agriculture
Language(s) - English
Resource type - Journals
eISSN - 1843-5386
pISSN - 1843-5246
DOI - 10.15835/buasvmcn-agr:5092
Subject(s) - ocimum , basilicum , carotenoid , chromatography , acetone , high performance liquid chromatography , chemistry , solvent , petroleum ether , methanol , analytical chemistry (journal) , extraction (chemistry) , organic chemistry , botany , food science , biology , essential oil
The analysis of carotenoids presents in inland Ocimum basilicum L. irradiated in a microwave field was performed in this study. The extracts of Ocimum basilicum L. were obtained with Ultraturrax in water: acetone (10 + 90, v/v) mixture, stirring 2 h. The extracts were analysed using a Jasco V-530 UV-VIS spectrophotometer and a Shimadzu HPLC equipped with PDA detector, for carotenoids identifying.
The HPLC analyses were performed on YMC C30 column, 5 μm, 250 x 4.6 mm, using a linear gradient of two different solvents mixtures A – methanol : methyl-tert-butyl-ether : water (81+15+4, v/v) and B with the sames component but in the follow range 6+90+4, v/v were the solvent mixtures used as mobile phase. The linear gradient was 1%B to 100%B in 90 min. The carotenoids identification was performed using the standard solutions.