Open AccessUse RAPD Technique for Characterization of Genotypical Variability of Maize Germplasm from SCDA Turda
Author(s) -
Rodica Pop,
Doru Pamfil,
Monica Hârţa,
Ioan Haș,
Iulia Francesca Pop
Publication year - 2010
Publication title -
bulletin of university of agricultural sciences and veterinary medicine cluj-napoca. agriculture
Language(s) - English
Resource type - Journals
eISSN - 1843-5386
pISSN - 1843-5246
DOI - 10.15835/buasvmcn-agr:5078
Subject(s) - rapd , dendrogram , upgma , germplasm , genetic diversity , biology , primer (cosmetics) , genotype , genetic variability , genetic variation , genetic distance , veterinary medicine , genetics , botany , chemistry , gene , population , medicine , demography , organic chemistry , sociology
Genetic analysis with RAPD markers has been extensively used to determine diversity among maize genotypes. The aim of the present study was to estimate genetic relationships among 70 genotypes, provided from SCDA Turda Cluj germplasm collection. RAPD analysis was performed with 14 decamer primers.
These primers generated, among the studied genotypes, a number of polymorphic bands comprised between 13 bands (OPA 04) and 7 bands (OPAL 20). The highest numbers of polymorphic bands were obtained with primer OPA 04, respectively 13 bands, following by OPO 12 (12 polymorphic bands), OPAB 11 and OPA 17 (11 polymorphic bands). Lowest number was obtained with primer OPAL 20, respectively 7 polymorphic bands. Genetic distances were established using Nei-Li coefficient and UPGMA dendrogram was constructed with RAPDistance 1.04 software. The built dendrogram shows phylogenetic relationships between genotypes analyzed.