Open AccessThe Biodegradative Ability Testing of the Microorganisms Isolated from Oil Contaminated Soils
Author(s) -
Laura Dobo,
Carmen Puia
Publication year - 2010
Publication title -
bulletin of university of agricultural sciences and veterinary medicine cluj-napoca. agriculture
Language(s) - English
Resource type - Journals
eISSN - 1843-5386
pISSN - 1843-5246
DOI - 10.15835/buasvmcn-agr:5017
Subject(s) - brevibacterium , achromobacter , biodegradation , microorganism , bacteria , microbacterium , alcaligenes , achromobacter xylosoxidans , arthrobacter , pseudomonas , flavobacterium , bacillus (shape) , biology , microbiology and biotechnology , bacilli , bacillus licheniformis , food science , bacillus subtilis , ecology , genetics
The bacteria and the fungi are the main microorganisms responsible of biodegradation of hydrocarbons in the oil. In the isolation of bacteria was used soil samples polluted with hydrocarbons. Soils were collected from two oil sites - Ploieşti and the Suplacu de Barcău. There were obtained a number of 41 pure bacterial cultures, on which were analyzed the following aspects: colony forming units, morphological characters (Gram staining and bacterial cell shape) and cultural characters of colonies (shape, pigmentation, gloss, profile, degree of transparency, and edge of colony).
Genera of bacteria identified were: Arthrobacter, Pseudomonas, Bacillus, Flavobacterium, Alcaligenes, Achromobacter, Brevibacterium, Clostridium, Corynebacterium, and Escherichia. Bacterial strains were tested on media with different concentrations of oil, aiming their biodegradation capacity of petroleum hydrocarbons. The microbial consortium was created of bacterial cultures which showed good and very good results, in the terms of their ability in the biodegradation of hydrocarbons.