Open AccessCollection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth
Author(s) -
Р. А. Илларионов,
О. В. Косякова,
Elena S. Vashukova,
Н. О. Юркина,
Margarita O. Shengelia,
Ю С Долгова,
Т. А. Сушко,
Д. Р. Еремеева,
М. С. Зайнулина,
М И Ярмолинская,
О. Г. Беспалова,
Andrey S. Glotov
Publication year - 2020
Publication title -
kardiovaskulârnaâ terapiâ i profilaktika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.158
H-Index - 16
eISSN - 2619-0125
pISSN - 1728-8800
DOI - 10.15829/1728-8800-2020-2708
Subject(s) - medicine , pregnancy , obstetrics , umbilical cord , data collection , abortion , blood collection , biobank , gynecology , bioinformatics , immunology , medical emergency , biology , statistics , genetics , mathematics
Aim . To create a collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth. Materialand methods. In order to standardize the sample collection, standard operation procedures have been developed with a step-by-step protocol for each research member at the clinical (collection of medical data and biological material) and laboratory (transportation, sample preparation, storage, quality control) stages. Results . As of October 1, 2020, the collection includes peripheral blood samples from 182 women. Whole blood, serum, plasma, buffy coat and urine were collected during pregnancy, and placenta and umbilical cord blood samples — during labor. Clinical and medical history data was obtained about each pregnant woman, which includes data on the woman’s health status, the course and outcome of pregnancy. An electronic catalog has been created with information on samples (data on clinical characteristics and the number of aliquots of each sample type). The quality control (assessment of DNA and microRNA) was carried out, which showed the compliance of the obtained samples with the quality criteria and the preservation of initial characteristics during long-term storage. On the basis of collection, a study has begun to assess the level of microRNA expression in various types of biomaterial, in order to search for early biomarkers of premature birth. Conclusion. The creation of a collection of samples from pregnant women is a significant groundwork for future fundamental and applied research in various fields of biomedicine. This collection may provide an in-depth study of the pathogenesis of various pregnancy complications and the development of new methods for their diagnosis and treatment.-