Open AccessSIBS triblock copolymers in cardiac surgery: <i>in vitro</i> and <i>in vivo</i> studies in comparison with ePTFE
Author(s) -
М. А. Резвова,
Е. А. Овчаренко,
П. А. Никишев,
Сергей Викторович Костюк,
Л. В. Антонова,
Т. Н. Акентьева,
Т. В. Глушкова,
Е. Г. Великанова,
Д. К. Шишкова,
Е. О. Кривкина,
К. Ю. Клышников,
Ю. А. Кудрявцева,
Л. С. Барбараш
Publication year - 2020
Publication title -
vestnik transplantologii i iskusstvennyh organov
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.137
H-Index - 5
eISSN - 2412-6160
pISSN - 1995-1191
DOI - 10.15825/1995-1191-2019-4-67-80
Subject(s) - biocompatibility , copolymer , in vivo , materials science , dispersity , polymer , polymer chemistry , biomedical engineering , biomaterial , chemistry , nanotechnology , medicine , composite material , biology , microbiology and biotechnology , metallurgy
Implantation of polymeric heart valves can solve the problems of existing valve substitutes – mechanical and biological. Objective : to comprehensively assess the hemocompatibility of styrene-isobutylene-styrene (SIBS) triblock copolymer, synthesized by controlled cationic polymerization in comparison with expanded polytetrafluoroethylene (ePTFE) used in clinical practice. Materials and methods . SIBS-based films were made by polymer solution casting method; in vitro biocompatibility assessment was performed using cell cultures, determining cell viability, cell adhesion and proliferation; tendency of materials to calcify was determined through in vitro accelerated calcification; i n vivo biocompatibility assessment was performed by subcutaneous implantation of rat samples; hemocompatibility was determined ex vivo by assessing the degree of hemolysis, aggregation, and platelet adhesion. Results . The molecular weight of synthesized polymer was 33,000 g/mol with a polydispersity index of 1.3. When studying cell adhesion, no significant differences (p = 0.20) between the properties of the SIBS polymer (588 cells/mm 2 ) and the properties of culture plastics (732 cells/mm 2 ) were discovered. Cell adhesion for the ePTFE material was 212 cells/mm 2 . Percentage of dead cells on SIBS and ePTFE samples was 4.40 and 4.72% (p = 0.93), respectively, for culture plastic – 1.16% (p < 0.05). Cell proliferation on the ePTFE surface (0.10%) was significantly lower (p < 0.05) than for the same parameters for SIBS and culture plastic (62.04 and 44.00%). Implantation results (60 days) showed the formation of fibrous capsules with average thicknesses of 42 μm (ePTFE) and 58 μm (SIBS). Calcium content in the explanted samples was 0.39 mg/g (SIBS), 1.25 mg/g (ePTFE) and 93.79 mg/g (GA-xenopericardium) (p < 0.05). Hemolysis level of red blood cells after contact with SIBS was 0.35%, ePTFE – 0.40%, which is below positive control (p < 0.05). Maximum platelet aggregation of intact platelet-rich blood plasma was 8.60%, in contact with SIBS polymer – 18.11%, with ePTFE – 22.74%. Conclusion . In terms of hemocompatibility properties, the investigated SIBS polymer is not inferior to ePTFE and can be used as a basis for development of polymeric prosthetic heart valves.