Open AccessInfluence of rat bone marrow mesenchymal stem cells to rat Langerhans islets viability during co-cultivation with microst ructured collagen‑containing hydrogel
Author(s) -
Natalia Valentinovna Baranova,
Л. А. Кирсанова,
З. З. Гоникова,
А. С. Пономарева,
V. I. Sevastianov
Publication year - 2018
Publication title -
vestnik transplantologii i iskusstvennyh organov
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.137
H-Index - 5
eISSN - 2412-6160
pISSN - 1995-1191
DOI - 10.15825/1995-1191-2018-3-54-63
Subject(s) - islet , mesenchymal stem cell , bone marrow , viability assay , incubation , andrology , staining , chemistry , biology , in vitro , microbiology and biotechnology , pathology , endocrinology , immunology , insulin , medicine , biochemistry
Aim . To research the rat bone marrow mesenchymal stem cells (MSC BM) effect to the viability of isolated rat OL during cultivation with microstructured collagen-containing hydrogel (BMCH). Materials and methods . Rat islets and MSC BM were isolated according to classical technique with some modifications. The experiments on the co- cultivation were carried out under standard conditions. Islet’s viability was determined by immunofluorescence staining. Results. TheOL cultivation with BMCH and MSCBM resulted in destructive changes of isletson the third day, and complete destruction of OL was observed on the seventh day. At the same time, OL cultured with BMCH remained viable for 14 days of incubation. Conclusion. Cultivation of OL with BMCH and MSC BM did not contribute to the long-term survival of the islets. Thus, there was no positive effect of MSC BM on the viability of pancreatic islets.