Open AccessISLET CELL CULTURES AS COMPONENT OF TISSUE-ENGINEERING CONSTRUCT OF PANCREAS
Author(s) -
Н. Н. Скалецкий,
Г. Н. Скалецкая,
Л. А. Кирсанова,
Natalia Valentinovna Baranova,
Г. Н. Бубенцова,
V. I. Sevastianov
Publication year - 2018
Publication title -
vestnik transplantologii i iskusstvennyh organov
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.137
H-Index - 5
eISSN - 2412-6160
pISSN - 1995-1191
DOI - 10.15825/1995-1191-2018-2-74-81
Subject(s) - islet , pancreas , biology , progenitor cell , immunohistochemistry , cell , cell culture , pathology , insulin , endocrinology , stem cell , microbiology and biotechnology , medicine , immunology , biochemistry , genetics
Aim: to develop methods for obtaining islet cell cultures for the purpose of their further use as a suitable component of the pancreatic tissue engineered construct. As a source of islet cell cultures, pancreas of newborn rabbits was used as an accessible and well-studied donor model. Materials and methods. For the obtaining of islet cell cultures, pancreas of 1–3-day-old newborn rabbits were used. Changes occurring during the cultivation of pancreatic tissue were recorded using an inverted microscope and a biostation. Morphological analysis of culture samples was carried out using histological and speci c immunohistochemical methods. The insulin-producing activity of the cultures was determined by enzyme immunoassay. Results. Three main types of cultures were obtained: isletlike organotypic, suspensionalcytotypic, and monolayered, consisting of progenitor cells. Greater morphological safety and adequate insulin-producing ability was revealed in oating islet-like cultures. Conclusion. According to their morphofunctional properties, otation islet-like cultures obtained from pancreas of the newborn rabbits can be used as basal cell component of the experimental model of the tissue engineered construct of the pancreas.