Open AccessINFLUENCE OF bFGF, SDF-1α, OR VEGF INCORPORATED INTO TUBULAR POLYMER SCAFFOLDS ON THE FORMATION OF SMALL-DIAMETER TISSUE-ENGINEERED BLOOD VESSEL IN VIVO
Author(s) -
Л. В. Антонова,
V. V. Sevost’yanova,
Anton G. Kutikhin,
Е. А. Великанова,
В. Г. Матвеева,
Т. В. Глушкова,
А. В. Миронов,
Е. О. Кривкина,
О. Л. Барбараш,
Л. С. Барбараш
Publication year - 2018
Publication title -
vestnik transplantologii i iskusstvennyh organov
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.137
H-Index - 5
eISSN - 2412-6160
pISSN - 1995-1191
DOI - 10.15825/1995-1191-2018-1-96-109
Subject(s) - stromal cell , vascular endothelial growth factor , in vivo , biomedical engineering , basic fibroblast growth factor , tissue engineering , angiogenesis , regeneration (biology) , blood vessel , growth factor , chemistry , vegf receptors , materials science , pathology , microbiology and biotechnology , cancer research , medicine , biology , receptor , biochemistry
Aim . To evaluate the potential synergistic effects of basic fi broblast growth factor (bFGF) and stromal cell-derived factor-1α (SDF-1α) complemented with VEGF compared to VEGF alone when being added into poly(3-hydroxybutyrateco-3-hydroxyvalerate) / poly(ε-caprolactone) vascular grafts implanted into rat abdominal aorta for 3, 6, or 12 months. Materials and methods . Utilizing emulsion electrospinning, we fabricated one-layer vascular grafts with either VEGF, bFGF, or SDF-1α, and two-layer vascular grafts with VEGF incorporated into the inner layer and bFGF and SDF-1α incorporated into the outer layer following structural evaluation, tensile testing, and in vivo testing using a rat abdominal aorta replacement model. Results . Grafts containing all three growth factors had a 100% primary patency rate. The combination of bFGF, SDF-1α and VEGF improved morphology and mechanical properties of the grafts. Furthermore, such combination of the bioactive factors promoted endothelialization compared to VEGF alone. In addition, bFGF induced a rapid formation of a SMC layer. Conclusion . Taking together, these fi ndings show that the incorporation of bFGF and SDF-1α into the vascular grafts in combination with VEGF enhances vascular tissue regeneration and provides a higher primary patency rate.