Open AccessMorphological evaluation of the quality of decellularized esophagus in rat
Author(s) -
Р. З. Накохов,
Е. А. Губарева,
Е. В. Куевда,
А. С. Сотниченко,
I. S. Gumenyuk,
А. Х. Каде,
G. M. Mogilnaya,
D. P. Puzanov
Publication year - 2017
Publication title -
vestnik transplantologii i iskusstvennyh organov
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.137
H-Index - 5
eISSN - 2412-6160
pISSN - 1995-1191
DOI - 10.15825/1995-1191-2017-2-98-103
Subject(s) - decellularization , extracellular matrix , esophagus , biomedical engineering , regeneration (biology) , scaffold , chemistry , anatomy , medicine , microbiology and biotechnology , biology , biochemistry
The main aim of our investigation was analysis and optimization of the existing protocols of esophagus decellularization in rats, identification of parameters affecting the quality of decellularization and intactness of the native properties of esophageal extracellular matrix (ECM). Materials and methods. We developed a modified decellularization protocol based on detergent-enzymatic method using sodium deoxycholate and DNAse. Results. Morphological evaluation of the obtained decellularized matrices demonstrated the absence of cells and cell elements while mechanical properties of ECM were preserved. Conclusion. The developed protocol of esophagus decellularization is a potential basis for obtaining of tissue-engineered esophagus scaffold constructions.