Open AccessANALYSIS OF T-HELPER SUBSETS OF PERIPHERAL BLOOD OF PATIENTS WITH CHRONIC HEPATITIS C EXPRESSING CHEMOKINE RECEPTORS CXCR3 AND CCR6 AND ACTIVATION MARKERS CD38 AND HLA-DR
Author(s) -
Дмитрий Сергеевич Елезов,
И. В. Кудрявцев,
Н. А. Арсентьева,
А. В. Семенов,
Е. В. Эсауленко,
В. В. Басина,
Арег А. Тотолян
Publication year - 2014
Publication title -
infekciâ i immunitet
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.137
H-Index - 5
eISSN - 2313-7398
pISSN - 2220-7619
DOI - 10.15789/2220-7619-2013-4-327-334
Subject(s) - cxcr3 , c c chemokine receptor type 6 , chemokine receptor , immunology , cd38 , cd8 , cd3 , medicine , biology , immune system , chemokine , microbiology and biotechnology , stem cell , cd34
. In case of hepatitis C the adequate adaptive immune response depends on T-helper lymphocyte subsets and recruiting specific T cells in the site of infection. The purpose of the study was to assess changes in subpopulations of T-helper cells bearing chemokine receptor CXCR3 and CCR6 and activation markers CD38 and HLA-DR in peripheral blood of patients with chronic hepatitis C (n = 19) and healthy donors (n = 32). The T-helper phenotype was assessed by flow cytometry using a combination of monoclonal antibodies HLA-DR-FITC/CD38-PE/CD3-ECD/CCR6-PE-Cy7/CXCR3-APC/CD4-APC-Cy7. It was demonstrated reduction of CD38+ subset, increase of CXCR3+ and CCR6+ subsets of T-helper cells in particular CD3+CD4+ CXCR3+CCR6+ cells and importance of joint determination of the chemokine receptors CXCR3 and CCR6 and the activation markers CD38 and HLA-DR compared to their separate analysis in chronic hepatitis C.