Open AccessTHE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES
Author(s) -
И. П. Баркова,
Ф. Г. Нагиева,
В Г Никулина,
А Н Лисаков
Publication year - 2014
Publication title -
infekciâ i immunitet
Language(s) - English
Resource type - Journals
eISSN - 2313-7398
pISSN - 2220-7619
DOI - 10.15789/2220-7619-2013-4-323-326
Subject(s) - rabies virus , rabies , virology , antigen , polyclonal antibodies , virus , biology , viral culture , cell culture , antibody , microbiology and biotechnology , immunology , genetics
. The rapid culture method for the indication of antigens of rabies virus has been improved. It allows to detect the antigen of rabies virus within 5 hours from the moment the cell cultures was infected. To achieve this aim it is necessary to use cell culture BHK-F, polyclonal antirabies FITC labelled immunoglobulin and direct method of membrane immunofluorescense. Applying of twofold freshly filtrated 3% paraformaldehyde as fixing solution allows to detect early virus proteins of rabies virus.