Open AccessStudies on correlations between immunophenotype and the indices of metabolic enzyme activity of blood lymphocytes in children with hypertrophy of the pharyngeal tonsils
Author(s) -
Л. М. Куртасова,
Н. А. Шакина,
Т. В. Лубнина
Publication year - 2020
Publication title -
medicinskaâ immunologiâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.133
H-Index - 6
eISSN - 2313-741X
pISSN - 1563-0625
DOI - 10.15789/1563-0625-soc-1806
Subject(s) - immunophenotyping , tonsil , cd3 , muscle hypertrophy , cd19 , peripheral blood , biology , peripheral blood mononuclear cell , medicine , endocrinology , cd8 , lymphocyte , palatine tonsil , pathology , immune system , immunology , flow cytometry , biochemistry , in vitro
The objective of our study was to evaluate correlation between the immune pheno-type and activity indices of NAD (P)-dependent dehydrogenases in peripheral blood lymphocytes in young children with hypertrophy of the pharyngeal tonsil (HPT). We have examined 57 children, 1-3 years of age, with hypertrophy of the pharyngeal tonsils (HPT). The control group consisted of 35 healthy children of the same age. The numbers of CD3+, CD4+, CD8+, CD19+, CD16+/56+ lymphoid cells in peripheral blood were determined by flow cytofluorimetry technique. Activity of NAD (P)-dependant dehydrogenases in peripheral blood lymphocytes was studied using bioluminescent method as described elsewhere (А. Savchenko, L. Suntsova, 1989). Correlation analysis has revealed an increase of positive correlations, a decrease of the correlation strength, and emergence of new connections between phenotype and activity indices of NAD (P)-dependent dehydrogenases in peripheral blood lymphocytes in children with hypertrophy of pharyngeal tonsils (HPT). Specific correlation patterns between the phenotype and activity indices of NAD (P)-dependent dehydrogenases in peripheral blood lymphocytes have been revealed in children with hypertrophy of pharyngeal tonsils (HPT).