Open AccessInfluence of sodium desoxyribonucleate on anti-infectious protection and hematopoiesis in patients with polytrauma (randomized prospective, double-blind, placebo-controlled study)
Author(s) -
Л. П. Пивоварова,
М. И. Громов,
А. Н. Тулупов,
В П Лапшин,
И. В. Осипова,
О. Б. Арискина,
А. В. Никитин,
М. Е. Малышев,
Е. Ва. Маркелова
Publication year - 2020
Publication title -
medicinskaâ immunologiâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.133
H-Index - 6
eISSN - 2313-741X
pISSN - 1563-0625
DOI - 10.15789/1563-0625-ios-1923
Subject(s) - medicine , gastroenterology , peripheral blood mononuclear cell , absolute neutrophil count , polytrauma , cd34 , immunology , surgery , neutropenia , biology , toxicity , biochemistry , in vitro , genetics , stem cell
The aim of the study was to evaluate the effect of sodium deoxyribonucleate on anti-infectious resistance and hematopoiesis in patients with polytraumas. A single-center study of sodium deoxyribonucleate effectiveness approved by the local Ethics Committee (protocol No. 4 05/18/2016), was conducted in 54 patients with polytrauma. The main group included 27 people, at the mean age of 39 (29-51) years old; ISS severity score, 26 (22-34). The comparison group comprised 27 people, mean age, 40 years old (26-53), mean ISS severity score was 25 points (20 to 29). The patients with randomly attributed even numbers were injected with 5 ml preparation from vials of even-numbered series, the patients with odd numbers were treated with preparation from the odd-numbered series. They were injected intramuscularly daily from day 1 to day 10 after the injury. Before treatment, as well as on days 8, 15 after injury, peripheral blood was examined for leukocyte, erythrocyte counts, hemoglobin, total protein, blood IL-6, CRP; proportion of CD117 + and CD34 + mononuclear cells, CD14 + monocytes, CD14 + granulocytes, HLA-DR + mononuclear cells, defensin + granulocytes. On the day +8, patients from the main group, against the comparison group showed an increase in lymphocytes, monocytes, CD117 + and CD34 + cell counts. Serum IL-6 and CRP were decreased in both groups of the patients to a similar degree. Terms of hospitalization in the main group were 32.8 days, against 39.6 in comparison group. The number of complications per 1 case was, respectively, 21 versus 39, thus being 1.8 times less than in comparison group. When developing complications, anemia (Hb < 90 g/l), or hypoproteinaemia (< 60 g/l) in the main group was, respectively, 2.5and 3.5-fold less than in the comparison group. Treatment with sodium deoxyribonucleate in polytrauma may promote migration of blood precursors to the bloodstream, increase anti-infectious properties of leukocytes, reduce duration of anemia and hypoproteinemia, number of complications and decrease the terms of hospitalization.