Open AccessEFFECT OF ERYTHROPOIETIN ON CYTOKINE PRODUCTION BY STEM CELLS
Author(s) -
А. П. Лыков,
М. А. Суровцева,
О. В. Повещенко,
Н. А. Бондаренко,
И. И. Ким,
А. М. Чернявский,
А. В. Фомичев
Publication year - 2019
Publication title -
medicinskaâ immunologiâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.133
H-Index - 6
eISSN - 2313-741X
pISSN - 1563-0625
DOI - 10.15789/1563-0625-2019-5-861-868
Subject(s) - erythropoietin , erythropoietin receptor , haematopoiesis , erythropoiesis , cytokine , medicine , receptor , stem cell factor , peripheral blood mononuclear cell , progenitor cell , endocrinology , stem cell , bone marrow , biology , chemistry , immunology , in vitro , microbiology and biotechnology , biochemistry , anemia
Erythropoietin (EPO) is mainly used to stimulate erythropoiesis. Its cytoprotective effects upon other cells of the human body and animals were recently shown, in particular, anti-apoptotic effect was observed. EPO effect upon the cells is mediated by interaction with erythropoietin receptor, with a complex forming a heterodimeric bond with β-common chain (CD131). In the present work, we studied the changes in erythropoietin receptor expression, and production spectrum of biologically active molecules in bone marrow mononuclear cells (BM-MNC) of patients with coronary heart disease. The flow cytometric assays showed that short-term incubation of BM-MNC with erythropoietin caused increased expression of the erythropoietin receptors on hematopoietic stem cells and tended to reduce the number of endothelial progenitor cells carrying the erythropoietin receptors. Solid-phase enzyme immunoassay in conditioned media from BM-MNC revealed that long-term (72 hours) exposure of BM-MNC to erythropoietin promoted increased production of IL-1β, PDGF-AB, and Epo, if compared to the basal production level (p < 0.05). Short-term incubation of BM-MNC with erythropoietin (60 minutes) caused a significant increase in the IL-1β, PDGF-AB and CXCL-12 / SDF-1α production levels, as well as significant reduction in the IL-10 production levels compared to the basal levels (p < 0.05).