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Blood Gas, Oxygen Saturation, pH, and Lactate Values in Elasmobranch Blood Measured with a Commercially Available Portable Clinical Analyzer and Standard Laboratory Instruments
Author(s) -
Gallagher Austin J.,
Frick Lorenz H.,
Bushnell Peter G.,
Brill Richard W.,
Mandelman John W.
Publication year - 2010
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/h10-012.1
Subject(s) - spectrum analyzer , blood lactate , gas analyzer , arterial blood , blood gas analysis , fish <actinopterygii> , oxygen saturation , biology , analytical chemistry (journal) , biomedical engineering , chromatography , oxygen , chemistry , medicine , environmental chemistry , computer science , blood pressure , fishery , endocrinology , telecommunications , heart rate , organic chemistry
Blood gas, pH, and lactate data are often used to assess the physiological status and health of fish and can often be most valuable when blood samples are analyzed immediately after collection. Portable clinical analyzers allow these measurements to be made easily in the field. However, these instruments are designed for clinical use and thus process samples at 37°C. A few studies have validated the use of portable clinical analyzers for assessing blood gases and acid—base profiles in teleosts, but equivalent data are not available for elasmobranchs. We therefore examined the relationship of blood gas, pH, and lactate values measured with an i‐STAT portable clinical analyzer with those measured using standard laboratory blood gas (thermostatted to 25°C) and lactate analyzers in samples taken from three species of carcharhiniform sharks. We found tight correlations ( r 2 > 0.90) between these methods for pH, p O 2 , p CO 2 , and lactate level values. We thus developed species‐specific equations for converting blood values measured with an i‐STAT portable clinical analyzer to those taken at 25°C. Additional studies need to address a wider range of temperatures and elasmobranch species.