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First Report of Yersinia ruckeri Biotype 2 in the USA
Author(s) -
Arias Cova R.,
OlivaresFuster Oscar,
Hayden Karl,
Shoemaker Craig A.,
Grizzle John M.,
Klesius Phillip H.
Publication year - 2007
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/h06-011.1
Subject(s) - yersinia ruckeri , biology , salmo , brown trout , microbiology and biotechnology , 16s ribosomal rna , trout , intergenic region , polymerase chain reaction , typing , gene , genetics , rainbow trout , genome , fishery , fish <actinopterygii>
A polyphasic characterization of atypical isolates of Yersinia ruckeri (causative agent of enteric redmouth disease in trout) obtained from hatchery‐reared brown trout Salmo trutta in South Carolina was performed. The Y. ruckeri isolates were biochemically and genetically distinct from reference cultures, including the type strain, but were unequivocally ascribed to the species Y. ruckeri , based on API 20E, VITEK, fatty acid methyl ester profiles, and 16S rRNA gene sequencing analysis. These isolates were nonmotile and unable to hydrolyze Tween 20/80 and were therefore classified as Y. ruckeri biotype 2. Genetic fingerprint typing of the isolates via enterobacterial repetitive intergenic consensus (amplified by polymerase chain reaction) and fragment length polymorphism showed biotype 2 as a homogeneous group distinguishable from other Y. ruckeri isolates. This is the first report of Y. ruckeri biotype 2 in the USA.