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Natural Infections of Wild Creek Chubs and Cultured Fathead Minnow by Chinese Grass Carp Reovirus (Golden Shiner Virus)
Author(s) -
Goodwin A. E.,
Nayak D. K.,
Bakal R. S.
Publication year - 2006
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/h05-029.1
Subject(s) - minnow , grass carp , biology , pimephales promelas , cyprinidae , carp , virology , zoology , virus , phoxinus , fishery , veterinary medicine , fish <actinopterygii> , medicine
Wild creek chubs Semotilus atromaculatus and farmed fathead minnow Pimephales promelas were cultured for viruses on epithelioma papulosum cyprini and fathead minnow cells. A syncytia‐forming virus was isolated from creek chubs and both healthy and moribund fathead minnow with skin and visceral lesions. The isolates were positive by a reverse‐transcriptase−polymerase chain reaction assay that amplifies segments 1 and 2 of Chinese grass carp reovirus (CGRV; also known as golden shiner virus and grass carp hemorrhagic virus). Sequencing of the amplicons showed that the isolates were 93–99% identical to each other and to North American CGRV isolates from grass carp Ctenopharyngodon idella and golden shiners Notemigonus crysoleucas and 87–93% identical to grass carp isolates from China. Homologies to other fish reoviruses were only 3–42%.