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Inhibitory Effects of Some Antibiotics on Activity of Carbonic Anhydrase from Rainbow Trout Erythrocytes in Vitro and in Vivo
Author(s) -
Hisar Olcay,
Hisar Şükriye Aras,
Küfrevioĝlu Öirfan,
Yanik Telat
Publication year - 2003
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/h03-005
Subject(s) - sulfamethoxazole , streptomycin , in vivo , trimethoprim , ampicillin , antibiotics , biology , biochemistry , in vitro , rainbow trout , enzyme , microbiology and biotechnology , pharmacology , chemistry , fishery , fish <actinopterygii>
Carbonic anhydrase (CA) from erythrocytes of rainbow trout Oncorhynchus mykiss was purified by affinity chromatography through a process comprising hemolysate preparation, sepharose‐ 4B‐l‐tyrosine‐sulfanylamide affinity gel chromatography, and dialysis. Then the effects of three antibiotics—sodium ampicillin, streptomycin, and trimethoprim plus sulfamethoxazole—on this enzyme were investigated in vitro and in vivo. Inhibition effects were determined in vitro by means of the Lineweaver–Burk and activity–antibiotic regression graphs. The dissociation constant of the enzyme inhibitor complex ( K i ) and 50% inhibitory values were 2.16 ± 0.40 and 1.87 mM for sodium ampicillin, 7.40 ± 0.310 and 5.21 mM for streptomycin, and 18.50 ± 0.35 and 9.74 mM for trimethoprim–sulfamethoxazole. The in vivo experiment demonstrated that CA was significantly inhibited by trimethoprim–sulfamethoxazole but not by sodium ampicillin and streptomycin.