Premium
Surface Disinfection and Removal of Adhesiveness from Rainbow Smelt Eggs
Author(s) -
Walker Abigail B.,
Ward Daniel,
Duclos Kristin,
Peters Michael,
Berlinsky David L.
Publication year - 2010
Publication title -
north american journal of aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 41
eISSN - 1548-8454
pISSN - 1522-2055
DOI - 10.1577/a09-047.1
Subject(s) - tannic acid , calcium hypochlorite , calcium , hatching , polyvinylpyrrolidone , biology , hydrogen peroxide , food science , zoology , chemistry , microbiology and biotechnology , chlorine , botany , biochemistry , organic chemistry
Abstract The survival of rainbow smelt Osmerus mordax embryos was evaluated after treating fertilized eggs with tannic acid (150, 300, 600, or 1,200 mg/L) in suspension for 10 min to remove adhesiveness or treating them with calcium hypochlorite (25, 50, 75, or 100 mg/L), polyvinylpyrrolidone iodine (PVP‐I; 25, 50, 75, or 100 mg/L), or hydrogen peroxide (H 2 O 2 ; 500, 1,000, 1,500, or 2,000 μL/L) for 15 min to achieve surface disinfection. Nonlethal concentrations of egg disinfectants were also evaluated for their germicidal effects by culturing disinfected eggs in sterile Bacto Tryptic Soy Broth. The hatching success of the eggs treated with tannic acid (at all concentrations) was not different from that of the controls. Disinfecting eggs with H 2 O 2 (all doses) and low doses of calcium hypochlorite (25–75 mg/L) or PVP‐I (25 mg/L) did not reduce embryo survival, but exposure to calcium hypochlorite and PVP‐I at higher concentrations (100 mg/L and 50, 75, or 100 mg/L, respectively) was lethal. In the first disinfection trial both H 2 O 2 and PVP‐I (25 mg/L) prevented bacterial growth, but only H 2 O 2 at 2,000 μL/L did so in the second trial.