Use of Hydrogen Peroxide to Improve Golden Shiner Egg Hatchability

The efficacy of hydrogen peroxide (H 2 O 2 ) in controlling fungal infections (saprolegniasis) in the eggs of golden shiner Notemigonus crysoleucas was assessed in two experiments. Golden shiner eggs were exposed in a 15‐min static bath (21°C) to 0, 50, 100, or 200 mg H 2 O 2 /L in the first experiment and to 0, 200, 400, and or 800 mg/L in the second experiment. All treatments were based on the amount of active ingredient (30% active ingredient concentration of H 2 O 2 ) in a single treatment. Three replicates of each concentration were used in each experiment. The objective was to determine the H 2 O 2 concentration that would result in optimum hatching success. The hatching rate significantly increased in each treatment level until 800 mg/L. The mean ± SE percent egg hatchability was 72.3 ± 8.55% and 68.2 ± 5.03% at 400 and 800 mg/L, respectively. Regression analysis revealed the peak treatment level to be between 400 and 800 mg/L.