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Stability of Allozyme and Mitochondrial DNA Markers among Three Year‐Classes of Lake Trout Propagated from Seneca Lake, New York
Author(s) -
Grewe Peter M.,
Krueger Charles C.,
Aquadro Charles F.,
May Bernie
Publication year - 1994
Publication title -
north american journal of fisheries management
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 72
eISSN - 1548-8675
pISSN - 0275-5947
DOI - 10.1577/1548-8675(1994)014<0467:soaamd>2.3.co;2
Subject(s) - trout , salvelinus , mitochondrial dna , biology , haplotype , zoology , genetics , genetic variation , allele , fishery , fish <actinopterygii> , gene
Lake trout Salvelinus namaycush representing three year‐classes (1988, 1989, and 1990) of gametes collected from adults captured in Seneca Lake, New York, were examined for allelic variation at 18 polymorphic loci and for mitochondrial DNA (mtDNA) haplotype variation, mtDNA was digested with the four restriction endonucleases Ava I, Bam H I, Hin f I, and Taq I. Analysis of allelic frequencies among these three year‐classes of the Seneca strain and a previously analyzed collection (1983 year‐class, also a wild egg taken from Seneca Lake) indicated temporal heterogeneity ( P < 0.01) at three loci: two for proline dipeptidase ( PEPD‐1,2* ) and one for malic enzyme (NADP + ) ( mMEP‐1* ). However, cluster analysis of genetic distances demonstrated that all four Seneca Lake samples exhibited a much lower level of differentiation than observed among other strains stocked into Lake Ontario. Mitochondrial DNA haplotype frequencies were not significantly different among the 1988, 1989, and 1990 year‐class samples, which supported the conclusion of genetic similarity among Seneca strain samples. Allozyme differences among Seneca collections did not significantly affect estimates by mixed‐stock analysis (MSA) of parental strain contributions to a mixture of wild fry from Lake Ontario. Based on these results, data from the four year‐classes could be pooled and used as Seneca strain baseline data for future MSA estimates.