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Mass‐Marking Otoliths of Larval and Juvenile Walleyes by Immersion in Oxytetracycline, Calcein, or Calcein Blue
Author(s) -
Brooks Ronald C.,
Heidinger Roy C.,
Kohler Christopher C.
Publication year - 1994
Publication title -
north american journal of fisheries management
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 72
eISSN - 1548-8675
pISSN - 0275-5947
DOI - 10.1577/1548-8675(1994)014<0143:mmoola>2.3.co;2
Subject(s) - calcein , stizostedion , oxytetracycline , juvenile , larva , fishery , biology , zoology , fish <actinopterygii> , ecology , biochemistry , antibiotics , membrane
Immersion of larval and juvenile walleyes Stizostedion vitreum for 6 h in a 500‐mg/L solution of oxytetracycline hydrochloride or Calcein resulted in fluorescent marks on 100% of the otoliths. Few marked fish (≤27.0%) were found after immersion in Calcein blue, Immersion of larvae at 15°C resulted in significantly higher mortality (57.7%) than at 10°C (20.9%). Mortality at 10°C was lowest for larvae immersed in oxytetracycline (9.0%) and greatest for those immersed in Calcein (41.6%). Juvenile mortality (1.3–32.7%) was correlated with high pond water temperatures during June (26.1°C) and August (32.2°C). All of the juveniles survived when immersed in any of the three chemicals in cases where pond water temperatures were less than 20°C at the time of collection.