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Amplification of a Piscirickettsia salmonis ‐like 16S rDNA Product from Bacterioplankton DNA Collected from the Coastal Waters of Oregon, USA
Author(s) -
Mauel M. J.,
Fryer J. L.
Publication year - 2001
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(2001)013<0280:aoapsl>2.0.co;2
Subject(s) - biology , polymerase chain reaction , 16s ribosomal rna , obligate , pathogen , ribosomal dna , microbiology and biotechnology , myxozoa , zoology , gene , genetics , phylogenetics , ecology , spore
Piscirickettsiosis is a disease observed in salmonids reared in net‐pens in the North and South Pacific and in the North Atlantic oceans. Piscirickettsia salmonis , the etiologic agent of piscirickettsiosis, is a gram‐negative, obligate, intracellular pathogen that replicates within cytoplasmic vacuoles. We describe here the detection, by dot‐blot hybridization, polymerase chain reaction, and sequence analysis, of a 16S rDNA sequence that is 99.5–98.2% similar to the 16S rDNA sequences of known isolates of P. salmonis.