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The Isolation of Edwardsiella ictaluri with a Limited Tolerance for Aerobic Growth from Channel Catfish
Author(s) -
Mitchell Andrew J.,
Goodwin Andrew E.
Publication year - 2000
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(2000)012<0297:tioeiw>2.0.co;2
Subject(s) - edwardsiella ictaluri , ictalurus , catfish , biology , microbiology and biotechnology , bacteriology , veterinary medicine , bacteria , isolation (microbiology) , fishery , fish <actinopterygii> , medicine , genetics
While culturing trunk kidneys from channel catfish Ictalurus punctatus with deep ulcerative lesions, we discovered strains of Edwardsiella ictaluri that could not be isolated under aerobic conditions. After passage of these bacterial isolates in an aerobic environment, minimal growth was observed. In thioglycolate broth, the passaged isolates had growth characteristics of bacteria with a limited tolerance for oxygen. Isolate S97‐133, from May 1997, was identified as E. ictaluri by the National Veterinary Services Laboratories, Diagnostic Bacteriology Laboratory, Ames, Iowa. The S97‐133 isolate and three others from 1994 were also definitively identified as E. ictaluri by the use of the monoclonal immuno dot‐blot technique. In all, eight isolates were obtained from channel catfish over an 8‐year period. All isolates were obtained during the months of May, June, July, and November.