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An Enzyme‐Linked Immunosorbent Assay (ELISA) for Detection of Antibodies to Channel Catfish Virus (CCV) in Channel Catfish
Author(s) -
Crawford Sara A.,
Gardner Ian A.,
Hedrick Ronald P.
Publication year - 1999
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1999)011<0148:aeliae>2.0.co;2
Subject(s) - ictalurus , catfish , biology , immunoassay , antibody , edwardsiella ictaluri , virus , ictaluridae , fish <actinopterygii> , microbiology and biotechnology , fishery , virology , immunology
An enzyme‐linked immunosorbent assay (ELISA) was developed for the detection of antibodies against channel catfish virus (CCV) in the serum of channel catfish Ictalurus punctatus. The ELISA was used to test sera from 20 waterborne CCV‐exposed juvenile channel catfish and 10 unexposed channel catfish for antibodies to CCV. Each serum was tested at a single dilution (1:10, volume:volume), and positive–negative cutoff values were established as the mean of negative sera plus either 2, 3, or 4 standard deviations. The resulting ELISA was highly reproducible and accurate with sensitivities and specificities of 100% and 90%, 95% and 90%, and 95% and 100%, respectively. Most fish seroconverted between 22 and 28 d after first exposure to CCV. This immunoassay is capable of handling large numbers of samples reliably and rapidly for detection and assessing prevalence of CCV exposure in channel catfish.