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Genomic Subtyping of Edwardsiella ictaluri Isolated from Diseased Channel Catfish by Arbitrarily Primed Polymerase Chain Reaction
Author(s) -
Bader Joel A.,
Shoemaker Craig A.,
Klesius Phillip H.,
Connolly Michael A.,
Barbaree James M.
Publication year - 1998
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1998)010<0022:gsoeii>2.0.co;2
Subject(s) - catfish , biology , edwardsiella ictaluri , polymerase chain reaction , subtyping , ictalurus , primer (cosmetics) , genetics , microbiology and biotechnology , gene , fishery , fish <actinopterygii> , computer science , programming language , chemistry , organic chemistry
Arbitrarily primed polymerase chain reaction (AP‐PCR) combined with an enterobacterial repetitive intergenic consensus PCR primer (ERIC II) was used to genomically subtype 20 isolates of Edwardsiella ictaluri. Nineteen isolates were derived from channel catfish Ictalurus punctatus (18 from the southeastern United States and an American Type Culture Collection reference strain), and 1 isolate was derived from a walking catfish Clarias batrachus from Thailand. From these isolates, four major subgroupings of E. ictaluri were observed. These subgroups were distributed with the following prevalences: subgroup 1, 55%; subgroup 4, 20%; subgroup 2, 15%; and subgroup 3, 10%. Biochemical analysis showed homogeneity among isolates and was not useful for discriminating between isolates.