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Antibacterial Properties of Tannic Acid and Related Compounds against the Fish Pathogen Cytophaga columnaris
Author(s) -
Zhao Guojing,
Chung KingThom,
Milow Kimberly,
Wang Wenxian,
Stevens S. Edward
Publication year - 1997
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1997)009<0309:apotaa>2.3.co;2
Subject(s) - tannic acid , gallic acid , methyl gallate , biology , microbiology and biotechnology , food science , biochemistry , botany , antioxidant
Tannic acid, gallic acid, and propyl gallate exhibited inhibitory activity as demonstrated by the agar dilution assay against Cytophaga columnaris (= Bacillus columnaris , Chondrococcus columnaris , Flexibacter columnaris , or Flavobacterium columnare ), a ubiquitous gliding fish pathogen, at 150, 275, and 300 μg/mL, respectively, in modified Shieh medium, at a low‐bacterial‐inoclum density of 10 3–4 colony‐forming units/mL. Methyl gallate was not effective at the highest concentration tested (500 μg/mL). The minimum inhibitory concentrations (MICs) of gallic acid, methyl gallate, and propyl gallate were lower in natural pond water than in modified Shieh medium, whereas the MIC of tannic acid was the same in both. Tannic acid, a polymeric compound with multiple hydroxyl groups, had a greater capacity for binding protein and glycogen by at least nine times that of the other test compounds. The results suggest that the hydroxyl group availability of tannins is essential for antibacterial activity.