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Characteristics of Peripheral Blood Cells from Rainbow Trout Evaluated by Particle Counter, Image Analysis, and Hemocytometric Techniques
Author(s) -
Huffman P A.,
Arkoosh M. R.,
Casillas E.
Publication year - 1997
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1997)009<0239:copbcf>2.3.co;2
Subject(s) - hemocytometer , rainbow trout , peripheral blood , coulter counter , particle counter , biology , white blood cell , peripheral , particle (ecology) , fish <actinopterygii> , blood cell , venous blood , anatomy , immunology , medicine , chemistry , fishery , ecology , microbiology and biotechnology , endocrinology , biochemistry , aerosol , organic chemistry
Hematological assessments of peripheral blood cells from rainbow trout Oncorhynchus mykiss were compared by using a hemocytometer, image analysis, and an electronic particle counter. Although statistical differences were noted, the diameters of white blood cells (WBCs) were comparable among the methods used, but lengths and widths of red blood cells (RBCs) were remarkably different among the three methods. Red blood cells were significantly smaller when the particle counter was used compared with sizes obtained by using the hemocytometer or image analysis. Counts of whole blood and isolated RBC concentrations were similar between the hemocytometer and the particle counter in the automatic calculation mode; yet they were significantly different with the manual calculation method, which used the raw data from the particle counter. White blood cell counts from peripheral whole blood could not be obtained with the particle counter; it converted all particle volumes into spherical equivalents and, thus, interpreted rainbow trout RBCs and WBCs to be similar in size, resulting in an inability to accurately count the number of WBCs in mixed‐cell populations. In addition, a lysing agent, which is commonly used to count WBCs in mammalian peripheral blood, did not completely remove all RBCs from samples of trout peripheral whole blood because of the presence of nuclei within the fish RBCs. These results indicate that assessing fish hematological characteristics with the particle counter may have limitations.