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Efficacy of a Modified Opercular Hypophysectomy Technique in Tilapia as Determined by Serum Cortisol Levels
Author(s) -
Kelly Anita M.,
Kohler Christopher C.,
Syska Scott R.
Publication year - 1996
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1996)008<0260:eoamoh>2.3.co;2
Subject(s) - hypophysectomy , nile tilapia , oreochromis , tilapia , biology , medicine , radioimmunoassay , endocrinology , fish <actinopterygii> , hydrocortisone , fishery , hormone
White tilapias, the hybrids of Nile tilapia Oreochromis niloticus × blue tilapia O. aureus , were rapidly hypophysectomized with a 2‐mm, high‐speed mototool cutter via an opercular approach. Two weeks post‐hypophysectomy, serum cortisol levels were nondetectable in 98 of the surviving test fish. The remaining 6 test fish and the nonhypophysectomized and shamoperated control fish had blood cortisol levels of 62–125 ng/mL. Necropsy confirmed complete and partial or unsuccessful hypophysectomies with results congruent with serum cortisol levels. The use of cortisol radioimmunoassay eliminates the need for visual observation and confirmation of pituitary removal at necropsy.