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Communications: Entry of 35 S‐Labeled Edwardsiella ictaluri into Channel Catfish
Author(s) -
Nusbaum Kenneth E.,
Morrison Edward E.
Publication year - 1996
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1996)008<0146:ceosle>2.3.co;2
Subject(s) - ictalurus , edwardsiella ictaluri , catfish , gill , bacteria , biology , microbiology and biotechnology , fish <actinopterygii> , ictaluridae , fishery , genetics
Channel catfish Ictalurus punctatus fingerlings were bathed for 1 h in water containing 5.6 × 10 6 Edwardsiella ictaluri /mL labeled with [ 35 S]methionine. Eight organs or tissues were collected from fish at varied intervals from 2 to 72 h after exposure; tissues were digested and radioactive disintegrations were measured in a liquid scintillation counter. A parallel study was conducted by using heat‐killed E. ictaluri from the same radiolabeled culture, Bacteria rapidly became associated with the gills (2 h) and persisted in or on the gill and liver (2–72 h). Fewer bacteria were detected in the trunk kidney, gut, and brain, Heat‐killed radiolabeled bacteria could not be detected in the fish, Our results suggest that E. ictaluri accumulates on and enters through the gill and that the gill should be considered as a route of entry. The absence of detectable heat‐killed bacteria indicates a need to reexamine strategies for fish vaccination with killed bacterins.