Premium
Growth Inhibition of Selected Aquatic Bacteria by Tannic Acid and Related Compounds
Author(s) -
Chung KingThom,
Zhao Guojing,
Stevens Edward,
Simco Bill A.,
Wei C. I.
Publication year - 1995
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1995)007<0046:giosab>2.3.co;2
Subject(s) - tannic acid , gallic acid , methyl gallate , propyl gallate , gallate , pseudomonas fluorescens , escherichia coli , microbiology and biotechnology , biology , chemistry , biochemistry , bacteria , food science , nuclear chemistry , botany , antioxidant , genetics , gene
Tannic acid, propyl gallate, methyl gallate, and gallic acid were tested for their inhibitory effects on selected aquatic microorganisms by the well assay technique. Tannic acid, propyl gallate, and methyl gallate in deionized water inhibited the growth of Aeromonas hydrophila , A. sobria , Edwardsiella iclaluri , E. tarda , Pseudomonas fluorescens , and Escherichia coli , but gallic acid did not. When 500‐μg/mL concentrations of these four compounds were tested in sterilized fish pond water at pH 7.0 and with a low bacterial inoculum of 10 3 –10 4 colony‐forming units (CFU) per milliliter, they inhibited the growth of P. fluorescens and (except for tannic acid) E. coli . Pseudomonas fluorescens inoculated at 10 3 –10 4 CFU/mL in pond water was inhibited by methyl gallate, propyl gallate, and tannic acid concentrations as low as 50 μg/mL, but a gallic acid contration of 100 μg/mL was required for inhibition. Escherichia coli was inhibited by methyl gallate and propyl gallate at 250 μg/mL and by gallic acid at 500 μg/mL, but it was not inhibited by tannic acid at concentrations up to 500 μg/mL in water of pH 7.0. Tannic acid (500 μg/mL) did inhibit E. coli growth at pH 4.5.