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Paddlefish Precipitating‐Antibody Reactions and in Vitro Macrophage Adherence with Larvae of Hysterothylacium dollfusi
Author(s) -
Ottinger Christoper A.,
Holloway Harry L.
Publication year - 1994
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1994)006<0303:pparai>2.3.co;2
Subject(s) - biology , larva , antibody , macrophage , in vitro , immune system , anisakidae , immunology , microbiology and biotechnology , fishery , ecology , biochemistry
Sera and macrophages of paddlefish Polyodon spathula were used to examine possible interaction between humoral and cellular components of the immune system and encysting larvae of Hysterothylacium dollfusi (Nematoda; Anisakidae). Sera, macrophages, and encysted larvae were obtained from paddlefish taken from the upper Missouri River drainage during spring spawning migrations. Sera from 6.8% of female paddlefish examined ( N = 44) in double‐immunodiffusion assays contained precipitating antibodies that reacted with homogenate prepared from H. dollfusi larvae. A significantly higher percentage (55.9%) of sera from male paddlefish examined ( N = 9) in these assays contained precipitating antibodies. Assays conducted in 1992 and 1993 showed that macrophages from paddlefish adhere to H. dollfusi larvae in vitro and that the time required for paddlefish macrophages to appear on the surface of larvae was significantly less for antibodyopsonized larvae than for nontreated larvae. Observations from 1993 also indicated that a heatlabile component of paddlefish serum may opsonize H. dollfusi larvae for macrophage adherence.