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Ciprofloxacin Treatment Eliminates Mycoplasma in Contaminated Channel Catfish Ovary Cells
Author(s) -
Hanson Larry A.,
Thune Ronald L.
Publication year - 1994
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1994)006<0082:ctemic>2.3.co;2
Subject(s) - biology , mycoplasma , catfish , mycoplasma hominis , cell culture , microbiology and biotechnology , ciprofloxacin , ovary , antibiotics , genetics , fish <actinopterygii> , fishery
A temperature‐sensitive mycoplasma that was present in the channel catfish ovary (CCO) cell line and in a thymidine kinase‐negative mutant of this cell line (CCOBr) was effectively eliminated from the cultures by adding ciprofloxacin, a 4‐fluoroquinolone, to the culture medium. Both cell lines have remained mycoplasma free after 3 years of continuous culture. A commercially available genetic probe to mycoplasma ribosomal RNA was used to monitor mycoplasma contamination and evaluate the effectiveness of the treatment.