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Apoptotic Death of Peripheral Leukocytes in Channel Catfish: Effect of Confinement‐Induced Stress
Author(s) -
Alford P. B.,
Tomasso J. R.,
Bodine A. B.,
Kendall C.
Publication year - 1994
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1994)006<0064:adopli>2.3.co;2
Subject(s) - catfish , ictalurus , apoptosis , biology , in vitro , lysis , andrology , peripheral blood , programmed cell death , fish <actinopterygii> , immunology , endocrinology , medicine , biochemistry , fishery
Channel catfish Ictalurus punctatus were stressed by net confinement for 0–12 h, and the percentages of apoptotic peripheral leukocytes were determined. The frequency of apoptosis was lower in stressed animals than in nonstressed controls, and the degree of reduction was dependent on the duration of stress. Leukocytes isolated from control fish were incubated in autologous plasma or plasma from stressed fish. The cells cultured in plasma from stressed fish showed decreased apoptosis, indicating a direct effect of stress on the individual cells rather than redistribution or cell lysis. Hydrocortisone (0.l mg/mL) had no effect in vitro on apoptosis in leukocytes. The mechanism, as well as the significance, of stress‐induced changes in apoptosis in circulating leukocyte populations remains to be explained.