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Detection of a Common Antigen among Renibacterium salmoninarum , Corynebacterium aquaticum , and Carnobacterium piscicola by the Western Blot Technique
Author(s) -
Bandín Isabel,
Santos Ysabel,
Barja Juan L.,
Toranzo Alicia E.
Publication year - 1993
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1993)005<0172:doacaa>2.3.co;2
Subject(s) - biology , western blot , microbiology and biotechnology , gel electrophoresis , antigen , polyacrylamide gel electrophoresis , bacteria , biochemistry , gene , enzyme , immunology , genetics
Cell surface extracts from isolates of Renibacterium salmoninarum , Corynebacterium aquaticum , and Carnobacterium piscicola were analyzed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and western blot. Although the electrophoretic analysis showed that the three bacterial species displayed different protein profiles, the immunoblot procedure with rabbit sera directed against R. salmoninarum ATCC 33209 and C. aquaticum ATCC 14665 revealed an immunoreactive band of approximately 57 kilodaltons (kDa) common to all isolates. These results suggest that any immunodiagnostic procedure relying on the detection of the 57‐kDa antigen of R. salmoninarum can result in false positive reactions.