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Communications: Isolation of Several Mycobacterium Species from Fish
Author(s) -
Lansdell W.,
Dixon B.,
Smith N.,
Benjamin L.
Publication year - 1993
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1993)005<0073:ciosms>2.3.co;2
Subject(s) - biology , isolation (microbiology) , microbiology and biotechnology , mycobacterium , staining , mycobacterium chelonae , fish <actinopterygii> , spleen , freshwater fish , zoology , bacteria , fishery , immunology , genetics
Several species of marine fish caught in the wild and of freshwater ornamental fish were used in this study. Infected organs (liver, spleen, and kidney) were sampled for mycobacteria. Decontaminated tissue samples were plated onto selective media for mycobacterial recovery. After initial isolation, fluorescent and acid‐fast staining techniques identified bacterial colonies to genus. Profiles of biochemical growth characteristics were used to further identify the isolates to species. Five species of Mycobacterium were identified: M. simiae , M. scrofulaceum , M. marinum , M. chelonae , and M. fortuitum . Of these, M. simiae and M. scrofulaceum have not been previously reported from fish. Tissue samples containing focal granulomatous lesions were prepared for electron microscopic examination.