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Isolation and Characterization of Flagella from Edwardsiella ictaluri
Author(s) -
Newton Joseph C.,
Triche Patricia L.
Publication year - 1993
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1993)005<0016:iacoff>2.3.co;2
Subject(s) - edwardsiella ictaluri , flagellum , biology , ictalurus , coomassie brilliant blue , catfish , microbiology and biotechnology , basal body , antiserum , bacteria , antigen , genetics , staining , fish <actinopterygii> , fishery
Flagella were isolated and purified by acid dissociation and by cell solubilization from 10 isolates of Edwardsiella ictaluri derived from channel catfish Ictalurus punctatus . The preparations were examined ultrastructurally and electrophoretically. Ultrastructurally, flagella isolated by the two methods were morphologically different. Flagellar filaments purified by acid dissociation were of various lengths, and hooks and basal bodies were not seen. When cell solubilization was used, flagellar filaments were more uniform in length, and hooks and basal bodies were evident. A flagellar sheath was not seen in either preparation. Electrophoretic (SDS‐PAGE) analysis of flagella isolated by either method showed two proteins with apparent molecular masses of 42 and 38 kilodaltons in gels stained with Coomassie Brilliant Blue.