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Development and Characterization of Monoclonal Antibodies against the Snakehead Rhabdovirus
Author(s) -
Kasornchandra J.,
CaswellReno P.,
Lannan C. N.,
Fryer J. L.
Publication year - 1992
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1992)004<0286:dacoma>2.3.co;2
Subject(s) - monoclonal antibody , snakehead , biology , immunofluorescence , virology , antibody , glycoprotein , microbiology and biotechnology , direct fluorescent antibody , fish <actinopterygii> , immunology , fishery
Monoclonal antibodies (MAbs) directed against the snakehead rhabdovirus (SHRV) were produced. These MAbs were characterized by immunofluorescence and neutralization tests, and by their ability to immunoprecipitate viral proteins. Of 15 MAbs developed, 9 were isotyped as IgG1 and 6 were IgG2a. Eight of the MAbs recognized the viral glycoprotein in an immuneprecipitation assay. Three of these, designated E1‐9A, P10C, and O10F, had neutralizing activity. By immunofluorescence, 12 MAbs showed good binding activity in SHRV‐infected epithelioma papulosum cyprini cells. In an indirect fluorescence assay, the MAbs gave varied staining patterns depending upon the viral structural proteins recognized.