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Description of a Virus Isolated from the Grouper Plectropomus maculatus
Author(s) -
ChewLim May,
Ngoh G. H.,
Chong S. Y.,
Chua H. C.Frederic,
Chan Y. C.,
Howe L. C.Josephine,
Lim E. W.
Publication year - 1992
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1992)004<0222:doavif>2.3.co;2
Subject(s) - grouper , biology , lethargy , virus , virology , spleen , lates , capsid , zoology , fishery , medicine , immunology , fish <actinopterygii>
The isolation of a virus from the liver and spleen of sick Plectropomus maculatus cultured in Singapore (and locally known as “red grouper”) is described. Clinical signs were inappetence and lethargy, followed by death 2–3 d later. The virus was isolated from spleen. It grew well in the locally established seabass fry cell line (derived from Asian seabass Lates calcarifer ) and in the bluegill fry cell line obtained from the American Type Culture Collection. The virus was shown to be ribonucleic acid, ether resistant, and able to tolerate heat treatment up to 56°C. Electron microscopy showed that the virus was a reovirus with a diameter of 67–72 nm, a double‐membrane capsid, and no envelope.