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Development and Evaluation of a Monoclonal‐Antibody‐Based Enzyme‐Linked Immunosorbent Assay for the Diagnosis of Renibacterium salmoninarum Infection
Author(s) -
Hsu HuiMin,
Bowser Paul R.,
Schachte John H.
Publication year - 1991
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1991)003<0168:daeoam>2.3.co;2
Subject(s) - biology , rainbow trout , monoclonal antibody , antigen , antibody , oncorhynchus , antibody titer , titer , agglutination (biology) , subclinical infection , microbiology and biotechnology , fishery , veterinary medicine , virology , fish <actinopterygii> , immunology , medicine
A monoclonal‐antibody‐based enzyme‐linked immunosorbent assay (ELISA) was developed for the diagnosis of bacterial kidney disease (BKD). This ELISA can detect Renibacterium salmoninarum antigen at concentrations as low as 0.05–0.1 μg/mL. During the 1988–1989 spawning season, 60 coho salmon Oncorhynchus kisutch , 60 chinook salmon O. tshawytscha , and 60 steelhead O. mykiss (Great Lakes rainbow trout) were caught and screened for BKD with the developed ELISA and a direct fluorescent antibody technique (FAT). Serum agglutination titers for R. salmoninarum were measured to determine any relationship between presence of antigen ( R. salmoninarum ) and humoral antibody to R. salmoninarum . Twelve of the coho salmon (20%), 48 of the chinook salmon (80%), and 7 of the steelhead (11.7%) were BKD‐positive according to the ELISA. Only one steelhead (1.7%) was BKD‐positive by FAT, whereas none of the coho salmon or chinook salmon were BKD‐positive. It was concluded that the monoclonal‐antibodybased ELISA was more sensitive than FAT. Antibody titers of these asymptomatic fish were variable. There was no correlation between antigen level and antibody titer.