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Rapid Fluorescent Antibody Tests for Infectious Hematopoeitic Necrosis Virus (IHNV) Utilizing Monoclonal Antibodies to the Nucleoprotein and Glycoprotein
Author(s) -
Arnzen Jeanene M.,
Ristow Sandra S.,
Hesson Colleen P.,
Lientz Joe
Publication year - 1991
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1991)003<0109:rfatfi>2.3.co;2
Subject(s) - nucleoprotein , biology , virology , monoclonal antibody , antibody , virus , glycoprotein , infectious hematopoietic necrosis virus , microbiology and biotechnology , immunology , fish <actinopterygii> , fishery , rainbow trout
A quick direct fluorescent antibody test incorporating a monoclonal antibody, 1NDW14D reacting with the nucleoprotein of infectious hematopoietic necrosis virus (IHNV), revealed the presence of the 1989 Dworshak isolates of the virus 8 h after chinook salmon embryo (CHSE‐214) cells were incubated with fish homogenates containing the virus. An indirect fluorescence assay was performed to compare the ability of the anti‐nucleoprotein antibody, 1 NDW 14D, with that of an anti‐glycoprotein antibody, 3GH 135L, to detect IHNV. Although the nucleoprotein of IHNV is synthesized before the glycoprotein, both antibodies detected the presence of virusinfected cells with equal efficiency when tested on several isolates of IHNV. Both antibodies stained caps of glycoprotein or nucleoprotein in the cytoplasm contiguous with the plasma membrane of infected cells within 6–8 h after infection.