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Detection of Antibodies Induced in Rainbow Trout by Different Aeromonas salmonicida Vaccine Preparations
Author(s) -
Hastings T. S.,
Ellis A. E.
Publication year - 1990
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1990)002<0135:doaiir>2.3.co;2
Subject(s) - aeromonas salmonicida , rainbow trout , antigen , immunogenicity , biology , antibody , microbiology and biotechnology , trout , lipopolysaccharide , immune system , immunology , virology , fish <actinopterygii> , fishery
Cell‐surface components and extracellular products (ECP) of Aeromonas salmonicida are candidates for the role of protective antigens in vaccines against furunculosis. However, the efficacy of experimental vaccines based on either cellular or extracellular antigens is disputed, and the immunogenicity in fish of many A. salmonicida antigens is unknown. In this study, rainbow trout Oncorhynchus mykiss and rabbits Oryctolagus cuniculus were immunized with whole killed A. salmonicida cells and with native and inactivated forms of ECP. Antibody responses were analyzed by western blotting. When immunized with whole killed cells, rabbits produced antibodies to A‐protein, lipopolysaccharide (LPS) core and O‐antigens, and at least 12 other protein antigens. In rainbow trout, antibodies were detected to A‐protein and LPS O‐antigens, but not to LPS core or other antigens. Antibodies to LPS were also detected in rabbits and rainbow trout immunized with ECP. However, whereas rabbits produced antibodies to at least 25 ECP proteins, antibodies to only 6 of these proteins were detected in the rainbow trout. Western blotting appears to be a useful technique for analyzing the immunogenicity of different antigens in multicomponent A. salmonicida vaccines .