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Unique Physicochemical Properties of the Occluded Penaeid Shrimp Baculoviruses and Their Use in Diagnosis of Infections
Author(s) -
Thurman R. B.,
Bell T. A.,
Lightner D. V.,
Hazanow S.
Publication year - 1990
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1990)002<0128:uppoto>2.3.co;2
Subject(s) - shrimp , biology , penaeus monodon , virology , stain , virus , autographa californica , microbiology and biotechnology , fluorescence microscope , staining , fluorescence , fishery , biochemistry , genetics , physics , quantum mechanics , spodoptera , gene , recombinant dna
Occlusion bodies in wet mounts of phloxine‐stained tissue squashes and paraffin sections of penaeid shrimp infected with Baculovirus penaei and Penaeus monodon ‐type baculovirus fluoresced when viewed with an epifluorescent microscope. Inclusions of hepatopancreatic parvo‐like virus, infectious hypodermal and hematopoietic necrosis virus, and Autographa californica nuclear polyhedrosis virus did not fluoresce. The use of phloxine stain (alone or as a component of an eosin preparation) and fluorescent microscopy may enhance detection of lowgrade infections by these disease‐causing agents.